LC–MS/MS analysis of puerarin and 18β-glycyrrhetinic acid in human plasma after oral administration of Samso-eum and its application to pharmacokinetic study |
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| Authors: | Seon Yu Lee Ji Hyun Jeong Bo Na Kim So Jung Park Yang-Chun Park Guk Yeo Lee |
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| Institution: | 1. Department of Korean Medicinal Resource, Development Korean Medicinal Herbs Standardization Team, National Development Institute of Korean Medicine, Anyang-myeon, Jangheung-gun, Jeollanam-do, Republic of Korea;2. Department of Internal Medicine, Daejeon Korean Medicine Hospital of Daejeon University, Daejeon, Republic of Korea |
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| Abstract: | The aim of this study was to confirm pharmacokinetic screening of multiple components in healthy Korean subjects after oral administration of Samso-eum and perform quantitation of active components in the human plasma. Thirteen potential bioactive components puerarin (PRR), daidzin, nodakenin, ginsenoside Rb1, 18β-glycyrrhetinic acid (18β-GTA), 6-shogaol, naringin, glycyrrhizin, hesperidin, platycodin D, naringenin, hesperetin, and 6-gingerol] were screened based on literature. The results showed that three analytes (daidzin, naringenin, and hesperetin) were detected in trace amounts. In addition, PRR and 18β-GTA were detected in human plasma after the oral administration of Samso-eum. In this study, a liquid chromatography–electrospray ionization-tandem mass spectrometry method was validated for the simultaneous determination of PRR and 18β-GTA in human plasma. This was the first study to evaluate pharmacokinetics of PRR and 18β-GTA after the usual oral dose of Samso-eum (30 g containing 102.48 mg PRR, 48.18 mg glycyrrhizin) in human subjects. |
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| Keywords: | 18β-glycyrrhetinic acid pharmacokinetics puerarin Samso-eum UHPLC–MS/MS |
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