LC/MS/MS determination and pharmacokinetic study of iridoid glycosides monotropein and deacetylasperulosidic acid isomers in rat plasma after oral administration of Morinda officinalis extract |
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| Authors: | Jingchang Tian Zhipeng Deng Xiujing Song |
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| Institution: | 1. Department of Urinary Surgery, the First Hospital of Qiqihaer City, Qiqihaer, China;2. Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan, China;3. Department of Respiratory, the First Hospital of Qiqihaer City, Qiqihaer, China |
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| Abstract: | Morinda officinalis is a famous traditional Chinese medicine containing iridoid glycoside compounds, such as monotropein and deacetylasperulosidic acid. The aim of the study was to develop a novel and sensitive liquid chromatography–tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of the two isomeric iridoid glycosides and then evaluate their pharmacokinetic properties in rats. Selected‐reaction monitoring mode was employed for quantification of two analytes in rat plasma. The calibration curves were linear over their respective concentration range with correlation coefficient >0.995 for both analytes. Precision for monotropein and deacetylasperulosidic acid ranged from 2.5 to 11.9% relative standard deviation, and the accuracy of two analytes was ?2.0–3.7 and ?6.4–10.7% relative error, respectively. This method was successfully applied in pharmacokinetic study after oral administration of M. officinalis extract in rats. The results provided a basis for further research on the bioactivity of M. officinalis. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | Morinda officinalis monotropein deacetylasperulosidic acid pharmacokinetics LC/MS/MS |
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