Metabonomic study on women of reproductive age treated with nutritional intervention: screening potential biomarkers related to neural tube defects occurrence |
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Authors: | Zhiting Jiang Qionglin Liang Yong Wang Xiaoying Zheng Lijun Pei Ting Zhang Yiming Wang Guoan Luo |
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Institution: | 1. School of Pharmacy, East‐China University of Science and Technology, Shanghai, 200237, People's Republic of China;2. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, People's Republic of China;3. WHO Collaborating Center For Research in Reproductive Health and Population Science, Institute of Population Research, Peking University, Beijing, 100084, People's Republic of China;4. Capital Institute of Pediatrics, Beijing, 100084, People's Republic of China |
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Abstract: | Nutritional intervention is effective in reducing the risk of neural tube defects (NTDs). To determine the effects of nutritional supplementation on human metabolism, a metabonomic study was carried out on 96 women of reproductive age. Subjects with nutritional intervention were given fortified wheat flour (containing folic acid, vitamin B1, vitamin B2, ferric sodium edetate and zinc oxide) for 8 months. Serum metabolic fingerprinting was detected via ultraperformance liquid chromatography in tandem with time‐of‐flight mass spectrometry (UPLC‐TOF MS), and data acquired was processed by multivariate statistical analysis. The result revealed a significant difference between the control and intervention group. Twenty potential biomarkers, including fructose 6‐phosphate, sphingosine 1‐phosphate, docosahexaenoic acid and hexadecanoic acid, were located and identified by the accurate mass measurement of TOF MS. These compounds are believed to be functionally related to anti‐oxidative competence in vivo. In conclusion, metabonomics study is a valuable approach in exploring the effect mechanism of nutritional intervention on NTD prevention. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | UPLC‐TOF MS metabolic fingerprinting biomarkers nutritional intervention |
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