Comparison of liquid chromatography-mass spectrometry interfaces for the analysis of polar metabolites of benz[a]pyrene |
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| Authors: | Robert Koeber R Niessner and Josep Maria Bayona |
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| Institution: | (1) Institute of Hydrochemistry, Technical University of Munich Marchioninistrasse 17, D-81377 Munich, Germany, DE;(2) Department of Environmental Chemistry, CID-C.S.I.C., Jordi Girona 18-26, E-08034 Barcelona, Spain, ES |
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| Abstract: | The performance of two liquid chromatography-mass spectrometry (LC/MS) interfacing techniques, thermospray (TSP) and atmospheric
pressure chemical ionization (APCI), for the analysis of benzoa]pyrene (BaP) metabolites (hydroxy, epoxy and quinone derivatives) was compared. Interface and detection parameters such as
source temperature, eluent composition or flow rate were optimized using negative ion mode. In TSP, the main ions are mostly
M]-, M−H2O]- or M+CH3COO]-, whereas APCI gives mainly the M]- and M−H]- ions. Quantification was carried out by flow injection. Calibration graphs were linear in the range of 10 ng to 1000 ng in
TSP and 0.1 ng to 10 ng in APCI. Detection limits were in the range of 1 ng to 20 ng in TSP and 0.002 ng to 0.2 ng in APCI.
The presence of BaP-1,6-dione, BaP-3,6-dione, and BaP-6,12-dione was confirmed in environmental samples of air particulate
matter.
Received: 6 January 1997/Accepted: 18 April 1997 |
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