磷酸盐缓冲溶液对邻二氮菲-Fe2+氧化法测定羟基自由基的影响

考察了不同浓度、不同体积的pH=7.4磷酸盐缓冲溶液对邻二氮菲-Fe2+氧化法检测羟基自由基体系的影响.将pH=7.4,浓度分别为0.1,0.15和0.2mol/L的磷酸盐缓冲溶液加入邻二氮菲-Fe2+体系中,随着各自缓冲溶液加入量的增加,其ΔA536不断减小,当加入量超过3mL时,ΔA536基本保持不变.采用抗坏血酸清除含有不同浓度磷酸盐缓冲溶液的邻二氮菲-Fe2+体系,实验结果表明,缓冲溶液浓度和加入量的不同,对于测定体系中清除羟基自由基的作用结果有影响.因此,邻二氮菲-Fe2+氧化法测定羟基自由基需明确体系中磷酸盐缓冲溶液的浓度及用量,在明确的统一体系中,才能进行有效的比较.

Influence of phosphate buffer solution on 10-phenanthroline-Fe2+ assay of hydroxyl radical

The effect of different concentration of phosphate buffer solution with pH=7.4 on hydrocarbonylation of 10-phenanthroline-Fe2+ to determine free radicals system was studied.The phosphate buffer solution of 0.1,0.15 and 0.2 mol/L were added to the system of 10-phenanthroline-Fe2+,with the increasing of buffer solution,ΔA536 decreased continually.When the buffer solution is more than 3 mL,ΔA536 remained stably.The system of 10-phenanthroline-Fe2+which contained the phosphate buffer solution with different concentration was eliminated by ascorbic acid.The result showed the effect of removal hydroxyl radicals changed with the concentration and addition of buffer solution.Therefore,the concentration and addition of phosphate buffer solution should be known on hydrocarbonylation of 10-phenanthroline-Fe2+ to determine free radicals system.The effectively comparison could be happened in one system.