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铜锌超氧化物歧化酶与精氨酸钴(Ⅱ)的相互作用
引用本文:郑学仿,高大彬,杨桦,胡皆汉.铜锌超氧化物歧化酶与精氨酸钴(Ⅱ)的相互作用[J].分子科学学报,2000,16(4):230-233.
作者姓名:郑学仿  高大彬  杨桦  胡皆汉
作者单位:1. 大连大学化学化工系,辽宁大连116622;大连理工大学生物工程系,辽宁大连116012
2. 大连大学化学化工系,辽宁大连116622
3. 大连理工大学生物工程系,辽宁大连116012
4. 中国科学院大连化学物理研究所,辽宁大连116023
基金项目:辽宁省科委自然科学基金资助项目! ( 972 0 67)
摘    要:采用VIS,ICP及酶活性测定等方法,研究了铜锌超氧化物歧化酶(Cu2Zn2SOD)与精氨酸钴(Ⅱ)(Co(Arg)n)的直接相互作用以及外加精氨酸钴(Ⅱ)的量、溶液pH值对此类相互作用的影响,结果发现:在水溶液中原酶活性中心处的Zn(Ⅱ)离子可被外加的精氨酸钴(Ⅱ)部分诱导、交换出来,而溶液中外加Co(Arg)n中的Co(Ⅱ)进行酶的活性中心,形成“Co-SOD”酶衍生物,并相应影响酶的催化活性,与此同时,外加精氨酸钴(Ⅱ)的量,溶液PH值对此类相互作用的进行有重要影响。

关 键 词:铜锌超氧歧化酶  精氨酸钴  相互作用  酶活测定
文章编号:1000-9035(2000)04-0230-04
修稿时间:2000年8月3日

Study on the interaction of copper-zinc superoxidedismutase with cobalt(Ⅱ) -arginine by spectral analysis
ZHENG Xue fang ,GAO Da bin ,YANG Hua ,HU Jie han.Study on the interaction of copper-zinc superoxidedismutase with cobalt(Ⅱ) -arginine by spectral analysis[J].Journal of Molecular Science,2000,16(4):230-233.
Authors:ZHENG Xue fang    GAO Da bin  YANG Hua  HU Jie han
Institution:ZHENG Xue fang 1,2,GAO Da bin 1,YANG Hua 2,HU Jie han 3
Abstract:The effect of changing the amount of extemal added Co(Arg)n and pH values onthe interaction of Cu2Zn2SOD with organic metal compound (Cobalt( lⅡ ) -Arginine) havebeen studied by means of ICP, VIS and measurement of enzyme activity. It has found thatin aqueous solution, there exists a direct interaction of the metal ions of the active center inthe metalloenzyme (Cu2Zn2SOD) with external added Co(Arg)n. As a result, part of themetal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives(Co(Ⅱ) - substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios(Cobalt(Ⅱ)-Arginine) and pH values and got some results under the effect of corresponding factors.
Keywords:Cu2Zn2SOD  Co(Arg) n (Ⅱ)  interaction
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