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Comparison of antioxidant capacities and antioxidant components of commercial bitter melon (Momordica charantia L.) products
Authors:Esin AKYÜZ  Sercan TÜRKOLU  Kevser S ZGEN BAKAN  Esma TÜTEM  Mustafa Reat APAK
Institution:1. Department of Chemistry, Faculty of Engineering, İstanbul University-Cerrahpaşa, İstanbul, Turkey ; 2. Sem Laboratory Equipments Marketing Industry and Trade Inc., İstanbul, Turkey
Abstract:In this study, the total phenolic contents and total antioxidant capacities of some commercial bitter melon products (powder, packaged powder, capsule, paste in olive oil), and of unripe and ripe fruits were determined by spectrophotometric and chromatographic methods. The total antioxidant capacities of unripe and ripe bitter melon samples, determined by using the CUPRAC (cupric reducing antioxidant capacity assay) and ABTS (2,2′-azino-bis(3-ethylbenzthiazolin-6-sulfonic acid))/HRP (horseradish peroxidase) methods, were 42.5 and 36.3 µmol TRE (Trolox equivalent) g–1, and 8.7 and 7.0 µmol TRE g–1, respectively. The TAC (total antioxidant capacity) order of the studied samples using the same 2 methods were determined as follows: capsule (CUPRAC value, 140.8; ABTS/HRP value, 143.6 µmol TRE g–1) > packaged powder (129.6; 126.1) > powder (52.3; 64.3) > unripe fruit (42.5; 36.3) > paste in olive oil (17.6; 14.4) > ripe fruit (8.7; 7.0). The order of phenolic content was found as follows: unripe fruit (193.2 µmol GAE (gallic acid equivalent) g-1) > capsule (162.0) > packaged powder (160.6) > powder (83.6) > paste in olive oil (38.3) > ripe fruit (14.6).
Keywords:Momordica charantia  bitter melon  total antioxidant capacity  total phenolic content  CUPRAC  ABTS  HPLC
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