Phycobiliproteins encapsulated in sol-gel glass |
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Authors: | Zhongping Chen D L Kaplan K Yang J Kumar K A Marx S K Tripathy |
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Institution: | (1) Biotechnology Division, U.S. Army Natick Research, Development and Engineering Center, 01760 Natick, Massachusetts;(2) Present address: Beckman Laser Institute, University of California, 92697 Irvine, CA, USA;(3) Center for Advanced Materials, University of Massachusetts Lowell, 01854 Lowell, Massachusetts, USA;(4) Center for Intelligent Biomaterials, Department of Physics, University of Massachusetts Lowell, 01854 Lowell, Massachusetts, USA;(5) Center for Advanced Materials, University of Massachusetts Lowell, 01854 Lowell, Massachusetts, USA;(6) Center for Intelligent Biomaterials, Department of Chemistry, University of Massachusetts Lowell, 01854 Lowell, Massachusetts, USA |
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Abstract: | Light transducing phycobiliproteins are encapsulated in optically transparent sol-gel matrices. Absorption and fluorescence spectroscopies are used to characterize the effect of the sol-gel encapsulation on the conformation and aggregation states of the three major phycobiliproteins present in phycobilisomes: phycoerythrin, phycocyanin, and allophycocyanin. It is found that the effects of sol-gel entrapment on the spectroscopic properties are significantly different for the three phycobiliproteins. The results indicate that phycoerythrin undergoes only minor change in its native structure when entrapped in sol-gel. However, significant changes in conformation and aggregation state occur when phycocyanin and allophycocyanin are entrapped in sol-gel matrices. A thin film of sol-gel encapsulated phycoerythrin is also coated on an optical fiber surface and strong fluorescence from the evanescent wave excitation is detected. The potential applications of sol-gel encapsulated phycobiliproteins in biosensors are discussed. |
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Keywords: | sol-gel glass phycobiliproteins biomaterial biosensor |
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