| A Novel and Convenient Assay for the Determination of OH Produced by Fenton Reaction |
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| 作者姓名： | Zhuo Bin YUAN Zhi Ru MA |
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| 作者单位： | Department of Chendstry，Graduate School，USTC，Academia Sinica，Beijing 100039 |
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| 摘 要： | Oxygenradicalsaswellas0theroxygen-derivedspeciesincludinglipidperoxides,havebeensuggestCdaspotentiallyimportantcausativeagents0fagingandvarioushumandi,...,i-3suchascancer,multiplescler0sis,Parkinson'sdisease,autoinununediseases,ischemia,anemia,seniledementiaandasbestosis.Am0ngthevari0usradicals,thehydroxylradical(OH)ispresumedtoplayacentralroleduetoitSstr0ngactivity'.TheimPortanceofremovingexcessiveactiveoxygenspeciesfromlivingorganismsisbecondngincreasinglyrec0gnized,t0getherwithagrowingint…
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A Novel and Convenient Assay for the Determination of OH Produced by Fenton Reaction |
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| Zhuo Bin YUAN, Zhi Ru MA.A Novel and Convenient Assay for the Determination of OH Produced by Fenton Reaction[J].Chinese Chemical Letters,1998(6). |
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| Authors: | Zhuo Bin YUAN Zhi Ru MA |
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| Abstract: | A novel, simple and convenient method for the determination of hydroxyl radicals isestablished. Hydroxyl radicals produced by Fenton reaction is trapped by spin trap reagent phenyl-t-butyl nitrone (PBN), and the free radical adduct of PBN can be detected by single sweeposcillopolarography, with its second order derivative cathodic wave at -0.52V vs SCE. Theoptimum experimental conditions for the detemination is discussed, and the scavenging effects ofsome compounds on OH was also studied. |
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| Keywords: | hydroxyl radicals Fenton reaction spin trap reagent PBN electrochemical method |
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