A quantitative colorimetric assay of H2O2 and glucose using silver nanoparticles induced by H2O2 and UV |
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Authors: | Yu Zhang Yan-Jun Zhang Xiao-Dong Xia Xiao-Qi Hou Cheng-Ting Feng Jian-Xiu Wang Liu Deng |
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Institution: | College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China |
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Abstract: | A simple spectrophotometric assay of H2O2 and glucose using Ag nanoparticles has been carried out. Relying on the synergistic effect of H2O2 reduction and ultraviolet (UV) irradiation, Ag nanoparticles with enhanced absorption signals were synthesized. H2O2 served as a reducing agent in the Ag nanoparticles formation in which Ag+ was reduced to Ag0 by O2? generated via the decomposition of H2O2 in alkaline media. On the other hand, photoreduction of Ag+ to Ag0 under UV irradiations also contributed to the nanoparticles formation. The synthesized nanoparticles were characterized by TEM, XPS, and XRD. The proposed method could determine H2O2 with concentrations ranging from 5.0 × 10?7 to 6.0 × 10?5 mol/L. The detection limit was estimated to be 2.0 × 10?7 mol/L. Since the conversion of glucose to gluconic acid catalyzed by glucose oxidase was companied with the formation of H2O2, the sensing protocol has been successfully utilized for the determination of glucose in human blood samples. The results were in good agreement with those determined by a local hospital. This colorimetric sensor thus holds great promises in clinical applications. |
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Keywords: | Silver nanoparticles Colorimetric assay H2O2 Glucose |
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