| Enzymatic Synthesis of a CCK-8 Tripeptide Derivative |
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| 作者姓名： | Li GUO Zi Min LU |
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| 作者单位： | Li GUO1*，Zi Min LU2，Heiner ECKSTEIN3 1West China School of Pharmacy，Sichuan University，Chengdu 610041 2Tongji Medical College，Huazhong Science & Technology University，Wuhan 430030 3Institute of Organic Chemistry，University of Tuebingen，72076 Tuebingen，Germany |
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| 摘 要： | The enzymatic synthesis of CCK-8 tripeptide derivative Phae-Met-Asp(OMe)-Phe-NH2 is reported.Starting with Phac-Met-OCam,we have successfully synthesized the target tripeptide with three free or immobilized enzymes, α-chymotrypsin,papain and thermolysin in reasonable yields.The key steps in this synthesis were the coupling of Phac-Met-OCam and H-Asp(OMe)2 to from Met-Asp peptide bond catalyzed by α-chymotrypsin and the selective hydrolysis of α-ester of Phac-met-Asp(OMe)2 catalyzed by papain.
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| 关 键 词： | CCK-8 三肽衍生物 选择性合成 木瓜蛋白酶 酶催化合成 |
Enzymatic Synthesis of a CCK-8 Tripeptide Derivative |
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| Li GUO,Zi Min LU,Heiner Eckstein.Enzymatic Synthesis of a CCK-8 Tripeptide Derivative[J].Chinese Chemical Letters,2003,14(2):167-168. |
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| Authors: | Li GUO Zi Min LU Heiner Eckstein |
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| Abstract: | The enzymatic synthesis of CCK-8 tripeptide derivative Phac-Met-Asp(OMe)-Phe-NH2 is reported. Starting with Phac-Met-OCam, we have successfully synthesized the target tripeptide with three free or immobilized enzymes, ?chymotrypsin, papain and thermolysin in reasonable yields. The key steps in this synthesis were the coupling of Phac-Met-OCam and H-Asp(OMe)2 to form Met-Asp peptide bond catalyzed by ?chymotrypsin and the selective hydrolysis of -ester of Phac-Met-Asp(OMe)2 catalyzed by papain. |
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| Keywords: | Enzymatic synthesis CCK-8 tripeptide derivative |
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