Point-of-care detection of Microcystin-LR with a personal glucose meter in drinking water source

In this study, a point-of-care sensing protocol has been reported for rapid and sensitive detection of Microcystin-LR (MC-LR) in water by personal glucose meter. The proposed immunosensor has been fabricated by using a primary antibody coated ZnFe₂O₄ nanoparticles to capture the target MC-LR. Consequently, the invertase@secondary antibody-conjugated graphene oxide-Au NPs can be immobilized for formating the sandwich immuno-complexes, which allowed for enzymatic conversion of sucrose to glucose. Thus, the concentration of MC-LR can be refelected by the converted glucose, which can be easily measured by the personal glucose meter (PGM). The PGM readout immunosensing method possessed good reproducibility and stability, which may have significant potential for other applications.