Sensitive detection of thyroid stimulating hormone by inkjet printed microchip with a double signal amplification strategy |
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Authors: | Beibei Yang Dongmei Liu Lina Zhu Yun Liu Xiaoning Wang Liang Qiao Weijia Zhang Baohong Liu |
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Institution: | Department of Chemistry, Shanghai Stomatological Hospital, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China; Department of Pharmacy, Qingdao Municipal Hospital, Qingdao 266001, China |
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Abstract: | An electrochemical immunosensor for sensitive detection of thyroid stimulating hormone (TSH) has been developed by using an inkjet printed microchip and based on a double signal amplification strategy using magnetic beads (MBs), alkaline phosphatase (ALP) and p-aminophenyl phosphate (pAPP) reaction. Differential pulse voltammetry (DPV), cyclic voltammogram (CV) and amperometric i–t curve (i–t) were employed to characterize the immunosensor. High sensitivity and good selectivity were observed. The detection linear range was from 0.01 μIU/mL to 10 μIU/mL, in which the peak currents increased along with the concentration. The detection limit was 0.005 μIU/mL at S/N = 3. The immunosensor was also applied for TSH detection in human serum with recoveries from 98.0% to 101.8% and relative standard deviations from 1.3% to 3.1%, demonstrating potential value in clinical diagnosis. |
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Keywords: | Electrochemical immunosensor Inkjet printed microchip Signal amplification Thyroid stimulating hormone Alkaline phosphatase |
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