A novel indirect inhibitive immunoassay for sulfamethoxazole |
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| Authors: | Ping Ping Tanga Zhao Feng Luob Ji Bao Caia Qing De Su a |
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| Institution: | a Department of Chemistry, University of Science and Technology of China, Hefei 230026, China;b Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China |
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| Abstract: | A new indirect inhibitive immunoassay using surface plasmon resonance (SPR) coupled with molecularly imprinted polymer (MIP) was developed. A sulfamethoxazole (SMX) MIP coating capillary was produced and used as an in-tube solid phase extraction (SPE) device. The MIP coating formed a nanometer film on the inner wall of the capillary. The anti-SMX mono-antibody was inhibited by SMX extracted by the MIP coating in a dose-dependent manner. The calibration curve was generated by linear fit over the range of 0.04-10.00 ng/mL. The limit of detection was 0.01 ng/mL. This method has high sensitivity and can be performed automatically. |
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| Keywords: | Sulfamethoxazole Molecularly imprinted polymer Surface plasmon resonance Indirect inhibitive immunoassay |
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