Current signal amplification strategies in aptamer-based electrochemical biosensor:A review |
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Authors: | Lei He Rongrong Huang Pengfeng Xiao Yuan Liu Lian Jin Hongna Liu Song Li Yan Deng Zhu Chen Zhiyang Li Nongyue He |
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Institution: | 1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, National Demonstration Center for Experimental Biomedical Engineering Education (Southeast University), Southeast University, Nanjing 210096, China;2. Department of Clinical Laboratory, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China;3. Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China |
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Abstract: | Due to their high specificity and affinity towards various targets,along with other unique advantages such as stability and low cost,aptamers are widely applied in analytical techniques.A typical aptamerbased electrochemical biosensor is composed of a aptamer as the biological recognition element and transducer conve rting the biologic interaction into electrical signals for the quantitative measure ment of targets.Improvement of the sensitivity of a biosensor is significantly important in order to achieve the detection of biomolecules with low abundance,and different amplification strategies have been explored.The strategies either employ nanomaterials such as gold nanoparticles to construct electrodes which can trans fer the biological reactions more efficiently,or attempt to obtain enha nced signal through multi-labeled carriers or utilize enzyme mimics to catalyze redox cycling.This review discusses recent advances in signal amplification methods and their applications.Critical assessment of each method is also considered. |
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Keywords: | Electrochemical Aptasensor Signal amplification Nanomaterials DNA nanotechnology |
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