| Polyethylenimine （PEI）-g-comb-poly（ethylene glycol）-transferrin（Ⅰ）： Tumor-targeted Vector for Gene Delivery In.vitro |
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| 作者姓名： | Gu Ping TANG Zhi Yu WANG |
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| 作者单位： | [1]College of Life-Science. ZheJiang University, Hangzhou 310028 [2]Institute of Materials Research ＆ Engineering, Singapore, 3 Research Link 117602 [3]The Second Hospital of Zhejiang University, Hangzhou 310006 |
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| 基金项目： | Acknowledgment This work was supported by the grants from the National High Biotechnology Development Program of China （2003AA216041）, and the National Key Basic Research Program of China （2004CB518802）. |
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| 摘 要： | The work described the synthesis and evaluation of PEI-g-comb-PEG-transferrin as a potential system for gene therapy in vitro. The MW of PEG was 10KDa, and PEI was 2KDa. Its structure was identified by NMR, FT-IR and TGA spectroscopy. MTT assay found that at concentration up to 4000 n mol/L of the polymer, cell viability was over 85%. The bio-character of polymer/DNA complex was characterized by agarose gel electrophoresis, ethidium bromide exclusion and zeta-potential assay. The polymer could retardate DNA at N/P ratio 3.0-3.5 （mol/mol）. The particle size of the polymer/DNA complex was less than 300 nm. Transfection efficiency of the complex was studied in COS7 and NT2 cell lines.
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| 关 键 词： | 聚乙烷基烯亚胺 PEI-g-冠-PE 聚乙二醇 铁传递蛋白 基因治疗 转染率 瘤 |
| 收稿时间： | 2005-04-24 |
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