基于氯化镁饱和溶液中固定化脂肪酶Lipozyme TL IM催化光皮树油脂合成生物柴油

基于氯化镁饱和溶液反应体系中,对采用固定化脂肪酶Lipozyme TL IM催化光皮树油脂转化为生物柴油的工艺进行了研究。考察了固定化脂肪酶Lipozyme TL IM催化光皮树油转酯化的工艺中甲醇的用量、固定化脂肪酶的添加量、摇床的转速和反应时间对生物柴油产率的影响。实验结果表明,采用氯化镁饱和溶液反应体系,在醇油摩尔比为3∶1,固定化酶Lipozyme TL IM用量为光皮树油质量的20%,摇床转速为150 r/min,反应8 h时,生物柴油产率最高,达到86.5%。与传统的三步甲醇醇解或者有机溶剂反应体系比较,采用的氯化镁饱和溶液体系的酶稳定性更好,反应效率更高,有效地解决了酶在甲醇中失活的问题,生产成本低,可成为生产生物柴油的新工艺。

Transesterification of Swida wilsoniana oil with methanol to biodiesel catalyzed by Lipozyme TL IM in MgCl 2-saturated solution

Transesterification of Swida wilsoniana oil with methanol to biodiesel was conducted by using the immobilized lipase Lipozyme TL IM as the catalyst in MgCl₂-saturated solution. The effects of alcohol/oil molar ratio, immobilized lipase loading, agitation speed, and reaction time on the transesterification were investigated. The yield of biodiesel reaches a maximum value of 86.5% after reaction for 8h under the optimal conditions: methanol/oil molar ratio of 3, 20% of Lipozyme TL IM based on Swida wilsoniana oil, and agitation speed of 150r/min. Compared with the traditional transesterification methods like using 3-step methanolysis or with tertiary butanol as solvent, the problem of immobilized lipase inactivation is solved effectively by using the MgCl₂-saturated solution. Moreover, the current process has potential for biodiesel production owing to its improvement in lipase stability, reaction efficiency, and production costs.