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Glycosyl azide—a novel substrate for enzymatic transglycosylations |
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| Authors: | Pavla Fialová Inmaculada Robina Petr Sedmera Lucie Petrásková-Hušáková |
| Institution: | a Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic b Department of Organic Chemistry, Faculty of Chemistry, University of Seville, Aptdo Correros 553, E-41071 Seville, Spain c Laboratory of High Performance Computing, Institute of Physical Biology USB and Institute of Systems Biology and Ecology AS CR, Zámek 136, CZ-373 33 Nové Hrady, Czech Republic |
| Abstract: | Glycosyl azides are new efficient donors for glycosidases. Their high water solubility facilitates transglycosylations with comparable or better yields than common O-glycosides. The azido group totally changes the β-GalNAc-ase/β-GlcNAc-ase ratio in β-N-acetylhexosaminidases (from the usual 0.3-1.0 to <0.01), contrary to all known aglycons. |
| Keywords: | β-N-Acetylhexosaminidase Enzyme catalysis Glycosyl azide Molecular modelling |
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