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Non-covalent modification of the active site of cytochrome P450 for inverting the stereoselectivity of monooxygenation |
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| Authors: | Takashi FujishiroOsami Shoji Yoshihito Watanabe |
| Institution: | a Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan b Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan |
| Abstract: | The enantioselectivity in the sulfoxidation of thioanisole catalyzed by cytochrome P450BSβ with a decoy molecule, a dummy molecule of the natural substrate, can be inverted by changing the structure of the decoy molecule. The methodology demonstrated herein shows the potential for controlling the stereoselectivity of biocatalysts without any mutagenesis. |
| Keywords: | Cytochrome P450 Stereoselectivity Sulfoxidation Protein modification Hydrogen peroxide Biocatalyst |
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