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Selective fluorescence and naked eye detection of histidine in aqueous medium via hydrogen bonding assisted Schiff base condensation
Authors:Sisir Lohar  Arnab Banerjee  Animesh Sahana  Sukanya Panja  Ipsit Hauli  Subhra Kanti Mukhopadhyay  Debasis Das
Institution:1. Department of Chemistry, The University of Burdwan, Burdwan 713104, West Bengal, India;2. Department of Microbiology, The University of Burdwan, Burdwan 713104, West Bengal, India
Abstract:Visible light excitable rhodamine B derivative (TARDHD) has been developed for fluorescence and naked eye detection of histidine in aqueous medium. TARDHD shows 45 fold fluorescence enhancement in the presence of histidine. It forms Schiff base with histidine and stabilizes via intra-molecular H-bonding. TARDHD can efficiently detect intracellular histidine.
Keywords:Visible light excitation  Naked eye  Histidine  Cell imaging  DFT
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