Hippurathydrolase,ein neues Enzym aus Mikroorganismen, 2. Mitt.: Enzymeigenschaften

Characteristic properties of the new enzymehippurate hydrolase are reported:

1. The Michaelis constant for hippurate hydrolysis is 5.5·10^?4 mole/l at 5° and pH 7.5. The specific activity of the purified enzyme preparation is 7.5 μmole/minute · mg enzyme protein at 37° and pH 7.5. The pH optimum is 7.5.
2. The effects of temperature on the reaction rate and on the stability of the enzyme were determined.
3. The effects of various inhibitors (HgCl₂, pCMB, EDTA, 8-hydroxyquinoline) and cations were studied. Participation of metal ions in the catalytic function ofhippurate hydrolase was not detectable.