Polymeric Sorbents for Bile Acids: II. Oligopeptide-Containing Resins with Quaternary Amine Groups |
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| Authors: | X X Zhu G R Brown L E St-pierre |
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| Institution: | 1. Department of Chemistry , University of Toronto , Erindale Campus, Mississauga, Ontario, Canada , L5L 1C6;2. Department of Chemistry , McGill University , 801 Sherbrooke St. W., Montreal, PQ, Canada , H3A 2K6 |
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| Abstract: | Abstract Polymeric sorbents for bile acids have been prepared by attaching lysine-containing peptide sequences onto a water-swellable polyamide resin, by solid phase peptide synthesis, and then attaching a terminal N,N-dimethyl glycine residue that was subsequently quaternized. The resins with relatively longer peptide sequences demonstrated a higher binding capacity, on a per active site basis, for bile acids in pH 7.4 aqueous buffer solutions at 20°C than cholestyramine and colestipol when tested under the same in vitro conditions. In solutions of low ionic strength, they also have a degree of specificity for bile acid anions. The resins have a higher binding affinity for cholic acid than for glycocholic acid, which indicates the importance of the hydrophobic interactions in the binding. |
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