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水溶性游离碱阳离子卟啉与核酸作用的光谱研究
引用本文:黄承志,李克安.水溶性游离碱阳离子卟啉与核酸作用的光谱研究[J].高等学校化学学报,1997,18(4):525-529.
作者姓名:黄承志  李克安
作者单位:北京大学化学与分子工程学院!北京,100871,北京大学化学与分子工程学院!北京,100871,北京大学化学与分子工程学院!北京,100871
基金项目:国家自然科学基金,国家教育委员会博士点基金
摘    要:研究了meso-四(对-三甲基氨基苯基)卟啉(TAPP)和meso-四(对-甲基吡啶基)卟啉(TMpyP-4)与核酸作用的电子吸收光谱、荧光光谱和共振光散射光谱的特性,电子吸收和荧光光谱研究表明,这两个水溶性阳离子卟啉与核酸的摩尔比(R)大于0.25时,核酸对卟啉的Soret带有减色效应和荧光猝灭效应;当R〈0.25时,核酸与卟啉作用形成新的荧光复合物,共振光散射光谱研究表明,核酸使TAPP的共振

关 键 词:核酸  荧光光谱  卟啉  TMpyP-4  TAPP  DNA  RNA

Spectroscopic Studies on the Interactions of Water-soluble Porphyrins with Nucleic Acids
HUANG Cheng-Zhi, LI Ke-An, TONG Shen-Yang.Spectroscopic Studies on the Interactions of Water-soluble Porphyrins with Nucleic Acids[J].Chemical Research In Chinese Universities,1997,18(4):525-529.
Authors:HUANG Cheng-Zhi  LI Ke-An  TONG Shen-Yang
Abstract:This paper presents the spectroscopic features of the interactions of two por-phyrins, meso-tetrakis (4-trimethylaminoniumphenyl )porphine (TAPP) and meso-tetrakls (N-methyl-4-pyridiniumyl)porphyrin (TMpyP-4 ) with nucleic aclds. Spectra involve electron ab-sorption, fluorescence and resonance light-scattering. The studies of absorption and fluores-cence show that the two porphyrins share common features when they interact with nucleicacids.That is, when the molar ratlo of porphyrins to nuclelc acids, R>0. 25, nuclelc acidshave hypochromicity and fluorescence quenching effect on the Soret bands of the two por-phyrins;when R<0. 25, new fluorescent products are formed. The difference of the interac-tions of the two porphyrins with nucleic acids can be distingushed by resonance light-scatter-ing spectra. Nucleic acids can strongly enhance the intensity of resonance light-scattering ofTAPP, but not of TMpyP-4.
Keywords:meso -Tetrakis(4-trimethylaminoniumphenyl)porphine  meso-Tetrakis(N-methyl-4-pyridiniumyl)porphyrin  Nucleic acids  Fluorescence spectroscopy  Resonance light-scat-tering spectroscopy
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