| 人CuZn-SOD的分子改造及在毕赤酵母中的表达 |
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| 引用本文： | 曲和之,杜姗姗,郝东云,张雷,黄露,王晓平.人CuZn-SOD的分子改造及在毕赤酵母中的表达[J].高等学校化学学报,2008,29(7):1390-1392. |
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| 作者姓名： | 曲和之 杜姗姗 郝东云 张雷 黄露 王晓平 |
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| 作者单位： | 吉林大学分子酶学工程教育部重点实验室,长春,130021 |
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| 摘 要： | 为了改善人CuZn-SOD的酶学性质, 在借鉴人细胞外超氧化物歧化酶的特性基础上, 利用分子生物学技术, 在人CuZn-SOD的C末端加入肝素亲和肽构建了人CuZn-SOD工程酶, 并在毕赤酵母中获得表达, 经纯化的工程酶具有较强的肝素亲和性.
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| 关 键 词： | 人铜锌超氧化物歧化酶 肝素亲和性 分子改造 工程酶 |
| 收稿时间： | 2007-09-28 |
Molecular Modification and Expression of Human CuZn Superoxide Dismutase in pichia pastries |
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| QU He-Zhi,DU Shan-Shan,HAO Dong-Yun,ZHANG Lei,HUANG Lu,WANG Xiao-Ping.Molecular Modification and Expression of Human CuZn Superoxide Dismutase in pichia pastries[J].Chemical Research In Chinese Universities,2008,29(7):1390-1392. |
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| Authors: | QU He-Zhi DU Shan-Shan HAO Dong-Yun ZHANG Lei HUANG Lu WANG Xiao-Ping |
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| Institution: | Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130021, China |
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| Abstract: | CuZn Superoxide dismutase(CuZn-SOD) is the enzyme that can catalyze the removal of superoxide radicals, which are generated in a variety of biological oxidations. It is a ubiquitous enzyme and provides a defense against oxygen toxicity. To dismutate superoxide radical effectively in and around vascular endothelial cells, we constructed a fusion gene encoding a hybrid SOD(namely HBSOD) consisting of human CuZnSOD and a C-terminal basic peptide that binds to heparin-like proteoglycans. The fusion gene was expressed successfully in pichia pastries. The purified HBSOD exhibited a normal SOD activity. The protein also possessed a high binding affinity to heparin proteoglycans. |
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| Keywords: | Human CuZn superoxide dismutase Heparin-affinity Molecular modification Engineering enzyme |
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