L-半胱氨酸衍生物配体交换手性色谱固定相

应用密闭微波萃取装置对芦荟中的有效成分芦荟甙进行了微波萃取研究，并利用透射电子显微镜对微波萃取机理进行了初步探讨,讨论了不同萃取剂、溶剂浓度、萃取时间和微波功率等对提取率的影响,在萃取剂为乙醇-水体系，溶剂（乙醇）体积分数为70％、萃取时间为4min及微波功率为340W的条件下，萃取效果最佳,与索氏提取及超声波萃取法相比，本法具有萃取速度快、提取率高及溶剂用量少等特点。

Microwave-assisted Extraction of Aloin from Fresh Aloe Leaves

A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl octyl ether and then coating it on YWG-C-{18} bonded stationary phase. The enantiomeric resolutions of some D,L-α-amino acids were achieved on CysCSP by using cupric acetate aqueous solution as the mobile phase, under the conditions of column temperature 20 ℃ and detection at UV 254 nm. The enantioselectivities α of D,L-α-amino acids separated were found to be in the range from 1.11 to 1.38 with the resolution R-s ranging from 1.1 to 2.8 and the column efficiency being from 5 000 to 9 000 n/m. The elution order of D-isomer before L-isomer was obersaved for all D,L-amino acids used.