新型荧光试剂1-(8-喹啉)-3-(2-吡啶)-三氮烯的合成及其分析应用

将具有荧光特性的8-氨基喹啉和吡啶类试剂结合, 并引入杂环三氮烯结构, 合成了新型荧光试剂1-(8-喹啉)-3-(2-吡啶)-三氮烯(QPyT). 其结构经元素分析、红外光谱和核磁共振谱证实. 研究结果表明, 在碱性介质中, 该试剂在λex/λem=216 nm/343 nm处产生强荧光, 并且能被Pb(Ⅱ)猝灭. 据此建立了QPyT测定Pb(Ⅱ)的新型荧光分析法. 该方法的线性范围为1.6×10-7~1.2×10-5 mol /L, 检测限为9.0×10-8 mol/L. 将其应用于水中Pb(Ⅱ)的测定, 结果令人满意.

Synthesis and Application of 1-(8-Quinoline)-3-(pyridyl)-triazene

Through combinination of 8-aminoquinoline with pydine through triazene group, a new fluorescent reagent 1-(8-quinoline)-3-(pyridyl)-triazene(QPyT) was synthesized. The product was confirmed by means of elemental analysis, 1H NMR and infrared spectra. Its excitation and emission wavelengths were 216 nm and 343 nm in a basic medium, respectively. This new reagent reacted with Pb(Ⅱ) in a slightly basic medium to form a 1∶1 complex, by which lead ion caused the fluorescence quenching of the new fluorescent reagent. The possible response mechanism of QPyT to Pb(Ⅱ) was discussed. The reaction showed a high selectivity and a high sensitivity for Pb(Ⅱ). The new fluorescent reagent was used to determined trace Pb(Ⅱ). The optimum analytical conditions for Pb(Ⅱ) assay were established. Under these conditions, the linear range of Pb(Ⅱ) was from 1.6×10-7 to 1.2×10-5 mol/L, with a detection limit 9.0×10-8 mol/L. The method is selective, sensitive and simple. It was used for the determination of trace Pb(Ⅱ) in water sample with satisfactory results.