姜黄素抑制金属离子诱导的丝素蛋白构象转变及其作用机理

以丝素蛋白(SF)为神经退行性疾病相关蛋白的模型蛋白, 分析了姜黄素(Curcumin)对Zn(Ⅱ)和Cu(Ⅱ)离子诱导的丝素蛋白构象转变的干预作用及其作用机理, 试图探讨姜黄素在神经退行性疾病中的预防与治疗作用. 结果表明, 姜黄素可通过与Cu(Ⅱ)和Zn(Ⅱ)离子络合, 干预金属离子诱导的丝素蛋白构象转变, 并且所形成的络合物Cu(Ⅱ)-Curcumin可抑制丝素蛋白的构象转变, 但是Zn(Ⅱ)-Curcumin络合物不具有这种能力. 因此, 基于姜黄素对于金属离子诱导的丝素蛋白构象转变具有良好的干预作用, 可以将姜黄素作为神经退行性疾病预防和治疗的潜在候选药物.

Inhibitory Effect of Curcumin on Metal Ions-induced Conformational Transition of Silk Fibroin and Its Mechanism

The inhibitory effect of curcumin on the Cu(Ⅱ) and Zn(Ⅱ)-induced conformation transition of silk fibroin(SF) as a model protein of neurodegenerative diseases, was investigated for aiming at investigating the potency of curcumin in the prevention and treatment of neurodegenerative diseases. The results indicate that curcumin can inhibit Cu(Ⅱ) and Zn(Ⅱ)-induced conformational transition of SF through chelating Cu(Ⅱ) and Zn(Ⅱ), meanwhile, Cu(Ⅱ)-curcumin complex also has ability to inhibit the conformation transition of SF, but Zn(Ⅱ)-curcumin complex does not. Therefore, based on those results about the effective interference of curcumin on Cu(Ⅱ) and Zn(Ⅱ)-induced conformation transition of SF, curcumin could be suggested as a potential agent for the prevention and treatment of neurodegenerative diseases.