纳米银/二甲基砜/聚乳酸-乙醇酸静电纺丝人工敷料的制备及生物评价

采用静电纺丝方法制备了纳米银(n-Ag)/二甲基砜(MSM)/聚乳酸-乙醇酸(PLGA)抗菌人工敷料.通过场发射扫描电子显微镜(ESEM)和能量离散X射线光谱(EDX)研究敷料的微观结构及表面元素组成.同时对敷料的力学性能、吸水性、细胞相容性及抗菌性进行测试,结果表明,该敷料内部纤维呈交叉的网格状结构,互相连结,随着n-Ag含量的增加,纤维的力学强度逐渐增大,吸水性能逐渐增强.当n-Ag质量分数达到1%以上时,敷料对革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性大肠杆菌具有良好的抗菌活性.细胞实验结果表明,当n-Ag含量在0.01%～10%之间时,敷料属无毒性或低毒性;当其含量小于1%时,有助于细胞的生长和增殖.因此,该敷料具有良好的细胞相容性和抗菌性能.

Preparation and Biological Evaluation of Electrospun MSM/PLGA Dressing Containing Nano-silver

Antibacterial dressings containing nano-silver powders(n-Ag)/dimethyl sulfoxide(MSM)/poly(lactide-co-glycolide)(PLGA) were prepared by electrospinning. Field emission scanning electron microscope(ESEM) and energy-dispersive X-ray spectroscopy(EDX) were applied for the investigation of the microscopic structure and surface elements of the dressings as well as mechanical properties, water absorption, cell compatibility and antibacterial function so that we could comprehensively evaluate the functionality and practicability of dressings. The results show that the fibers of dressing is a crossing grid-like structure, interconnected, and gradually enhenced the mechanical strength of fibers with increasing of n-Ag content, as well as water absorption ability. When the n-Ag content is more than 1%(mass fraction), the dressings show a good antibacterial ability against the Gram-positive staphylococci and Gram-negative Escherichia coli. Cell experimental results show that when the content of n-Ag is between 0.01%—10%, the dressings are non-toxic or low toxicity; when the content of n-Ag is less than 1%, it contributes to the growth and proliferation of cells. Therefore, we conclude that the dressing has a good biocompatibility and antibacterial properties, and has broad application prospects in a functional medical dressings research and industrial area.