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| 基于有序生物膜的电容型层胶粘蛋白新型免疫传感器 | |
| 引用本文： | 江德臣,唐佳,刘宝红,沈先荣,张松,杨原,李爱珍,黄宜平,孔继烈.基于有序生物膜的电容型层胶粘蛋白新型免疫传感器[J].高等学校化学学报,2003,24(1):46-48. |
| 作者姓名： | 江德臣 唐佳 刘宝红 沈先荣 张松 杨原 李爱珍 黄宜平 孔继烈 |
| 作者单位： | 1. 复旦大学化学系,上海,200433 2. 上海海军医药研究所,上海,200433 3. 复旦大学微电子系,上海,200433 |
| 基金项目： | 国家自然科学基金 (批准号 :39970 195 ,2 990 5 0 0 1和 2 0 0 0 5 0 0 2 )，长春应用化学研究所电化学国家重点实验室、上海市青年科技启明星计划资助，复旦大学三年行动规划“交叉项目资助计划”资助 |
| 摘 要： | 免疫传感器以其高度特异性抗原抗体结合反应的特点 ,可直接、快速而灵敏地测定抗原或抗体分子而被广泛应用于临床医学以及环境污染物检测等领域 1] .目前 ,检测抗原抗体结合的无标志方法主要有石英晶体微天平 2 ] 、表面等离子共振 3 ] 、界面电容 4 ] 及原子力显微镜 5] |
| 关 键 词： | 层胶粘蛋白 免疫传感器 自组装 电位阶跃 |
| 文章编号： | 0251-0790(2003)01-0046-03 |
| 收稿时间： | 2002-01-06 |
A Novel Capacitive Immunosensor For Direct Detection of Laminine with a Potentiostatic Step |
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| JIANG De-Chen ,TANG Jia ,LIU Bao-Hong ,SHEN Xian-Rong ,ZHANG Song ,YANG Peng-Yuan ,LI Ai-Zhen ,HUANG Yi-Ping ,KONG Ji-Lie.A Novel Capacitive Immunosensor For Direct Detection of Laminine with a Potentiostatic Step[J].Chemical Research In Chinese Universities,2003,24(1):46-48. | |
| Authors: | JIANG De-Chen TANG Jia LIU Bao-Hong SHEN Xian-Rong ZHANG Song YANG Peng-Yuan LI Ai-Zhen HUANG Yi-Ping KONG Ji-Lie |
| Institution: | JIANG De-Chen 1,TANG Jia 1,LIU Bao-Hong 1,SHEN Xian-Rong 3,ZHANG Song 1,YANG Peng-Yuan 1,LI Ai-Zhen 2,HUANG Yi-Ping 2,KONG Ji-Lie 1 * |
| Abstract: | Laminine(Ln) has been identified as an important marker of liver fibrosis or cirrhosis. The Ln immunosensor was formed with thioglycolic acid to produce a self-assembled monolayer on the gold electrode surface. The monoclonal Ln antibody was orderly immobilized on the modified gold surface covalently using EDC/NHS. An unlabeled antigen combined on the antibody could reduce the capacitance of the electrode, which was evaluated by the potentiostatic method. The measurement was performed in microseconds. Ln antigen could be determined in the range of 2-50 ng/mL. The quick response made the immunosensor a promising clinical screening method. |
| Keywords: | Laminine Immunosensor Self-assembly Potentiostatic step |
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