| 基于酶催化反应的核酸定量新方法 |
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| 引用本文： | 魏玲,杨黄浩,陈小兰,曲会英,许金钩.基于酶催化反应的核酸定量新方法[J].高等学校化学学报,2003,24(1):43-45. |
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| 作者姓名： | 魏玲 杨黄浩 陈小兰 曲会英 许金钩 |
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| 作者单位： | 1. 现代分析科学教育部重点实验室, 厦门大学化学系, 厦门 361005;
2. 新疆昌吉师范专科学校, 昌吉 831100 |
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| 摘 要： | 近年来 ,将染料自缔合或诱导缔合用于核酸定量测定备受关注 1～ 3 ] .但是将酶与染料的缔合用于核酸定量测定尚未见报道 .氯化血红素 (hemin)可作为辣根过氧化物酶 (HRP)的模拟酶 ,能催化 H2 O2氧化对 -羟基苯乙酸 (p- HPA)生成荧光产物——联二对 -羟基苯乙酸的反应 4 ,5] .由于 hemin在碱性介质中是阴离子化合物 ,能与阳离子化合物如阿尔新蓝 (Alcian Blue 8GX)发生缔合作用 ,从而使自身的催化性质被抑制 .当加入带负电荷的脱氧核糖核酸 (DNA)时 ,由于阿尔新蓝与 DNA的强烈作用使hemin与阿尔新蓝的缔合物被破坏 ,hemin的催化活…
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| 关 键 词： | 酶催化 离子缔合 核酸 荧光分析 |
| 文章编号： | 0251-0790(2003)01-0043-03 |
| 收稿时间： | 2001-10-26 |
A Novel Method for the Determination of Nucleic Acids Based on Enzyme Catalyzed Fluorescence Reaction |
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| WEI Ling ,YANG Huang-Hao ,CHEN Xiao-Lan ,QU Hui-Ying ,XU Jin-Gou.A Novel Method for the Determination of Nucleic Acids Based on Enzyme Catalyzed Fluorescence Reaction[J].Chemical Research In Chinese Universities,2003,24(1):43-45. |
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| Authors: | WEI Ling YANG Huang-Hao CHEN Xiao-Lan QU Hui-Ying XU Jin-Gou |
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| Institution: | 1. Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, China;
2. Department of Chemistry, Changji Teacher's Special College, Changji 831100, China |
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| Abstract: | A novel fluorimetric method has been developed for the rapid determination of ncleic acids, based on that the catalytic activity of hemin is decreased due to the ion-association with Alcian blue 8GX and recovered when nucleic acids are added to the system. The change in the catalytic activity of hemin results in the fluorescence change of the hemin-catalyzed fluorogenic reaction system of p-hydroxyphenylacetic acid(p-HPA) and H 2O 2 and the fluorescence change of the system is proportional to the quantities of nucleic acids existing. The corresponding detection limits of the method are 0 91 ng/mL for CT DNA and 7 5 ng/mL for yeast RNA. |
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| Keywords: | Enzyme catalyst Ion-association Nucleic acid Fluorescent analysis |
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