通过三嗪荧光探针的多氢键作用识别胸腺嘧啶

发展新型光学受体分子, 研究其对核酸碱基的选择性识别不仅有助于了解生物体内分子作用和转化的机理, 同时对发展新的生物分子检测方法和研制新药等都具有重要意义. 然而, 由于核酸碱基的性质相近, 因此实现单一碱基的选择性识别较难1]. 氢键是一种重要的分子间相互作用力, 在生物化学和分子药理学研究中, 尤其在生物大分子的三维结构中起着重要的作用2,3].

Recognition of Thymine by Triazinyl Fluorescence Probe Through the Formation of Intermolecular Multi-hydrogen Bonds

In this work, a novel long-wavelength fluorescence probe, 3-(4-chloro-6-methylamino-1,3,5-triazinylamino)-7-dimethylamino-2-methylphenazine, was synthesized by the reaction of neutral red and methylamine subsequently with cyanuric chloride, and its recognition behavior for thymine together with its spectroscopic properties in different solvents was studied. The results show that the fluorescence probe can be selectively quenched by thymine instead of guanine, suggesting that the fully complementary hydrogen bonding plays a key role in such a recognition process.