人工细胞外基质对血管内皮细胞生存的影响

通过物理吸附方法, 利用胶原、 聚赖氨酸和融合蛋白VEGF-Fc对聚苯乙烯培养板表面进行改性, 以研究细胞外基质材料对血管内皮细胞的影响. 结果表明, 3种蛋白显著提高了聚苯乙烯表面的亲水性. 内皮细胞的黏附、 增殖、 细胞骨架蛋白染色和血管性血友病因子(vWF)免疫染色实验结果表明, 胶原、 聚赖氨酸和VEGF-Fc基质均能有效提高血管内皮细胞的黏附, 其中胶原可与VEGF协同作用促进内皮细胞分化表型的表达; VEGF-Fc基质兼具了VEGF的生物学活性, 可促进内皮细胞的黏附和增殖以及vWF功能性蛋白的表达. 本研究为诱导材料表面内皮化和血管新生的生物活性材料的设计开发提供了新思路.

Effects of Artificial Extracellular Matrixes on the Survival of Vascular Endothelial Cells

In order to study the impact of extracellular matrix material on endothelial cells, collagen, poly-lysine and fusion protein VEGF-Fc were immobilized on the polystyrene cell culture plate by physical adsorbing, respectively. The three proteins significantly improved the hydrophilic of the polystyrene surface. Meanwhile, the cell adhesion, proliferation and the expressions of the cytoskeletal protein and von Willebrand factor(vWF) were investigated. The results showed that these matrixes effectively improved the adhesion of Human umbilical vein endothelial cells(HUVECs). And HUVECs cultured on fusion protein VEGF-Fc matrix without extra VEGF in the culture medium could also maintain and prolong the bioactivities of cell proliferation and vWF expression. Furthermore, HUVECs cultured on collagen and fusion protein matrixes expressed abundant stress fibers and filopodia. Thereby, the collagen and fusion protein VEGF-Fc matrixes constructed in this work provided suitable environment for HUVECs. Those results will provide a theoretical basis on achieving the endothelialization and improving the angiogenesis of implanted materials.