| 过氧化氢酶荧光分析法及其在海洋水生生物酶活力测定中的应用 |
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| 引用本文： | 毛玉霞,郑洪,郭祥群,陈荣,郑微云.过氧化氢酶荧光分析法及其在海洋水生生物酶活力测定中的应用[J].高等学校化学学报,2002,23(10):1864-1867. |
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| 作者姓名： | 毛玉霞 郑洪 郭祥群 陈荣 郑微云 |
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| 作者单位： | 1. 厦门大学化学系, 教育部现代分析科学重点实验室;
2. 海洋学系;
3. 环境科学研究中心;厦门 361005 |
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| 基金项目： | 福建省自然科学基金 (批准号 :D992 0 0 0 1)资助 |
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| 摘 要： | 建立了一种简便、灵敏的测定过氧化氢酶的方法.方法的线性范围为1.7×10-3～1.7×10-2U/mL,检测限(LD=3S0/S)为8.5×10-4U/mL.将其用于海洋生物样品中过氧化氢酶活力的测定,结果令人满意.
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| 关 键 词： | 过氧化氢酶测定 喹啉 荧光分析 Fenton反应 生物样品 |
| 文章编号： | 0251-0790(2002)10-1864-04 |
| 收稿时间： | 2001-06-13 |
Studies on the Fluorometric Method for Determining Activity of Catalase and Its Application to Marine Biosamples |
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| MAO Yu-Xia ,ZHENG Hong ,GUO Xiang-Qun ,CHEN Rong ,ZHENG Wei-Yun.Studies on the Fluorometric Method for Determining Activity of Catalase and Its Application to Marine Biosamples[J].Chemical Research In Chinese Universities,2002,23(10):1864-1867. |
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| Authors: | MAO Yu-Xia ZHENG Hong GUO Xiang-Qun CHEN Rong ZHENG Wei-Yun |
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| Institution: | 1. Departm ent of Chemistry and the Key Laboratory of Analytical Science of MOE;
2. Department of Oceanography;
3. The Enviromental Science Research Center, Xiam en U niversity, Xiamen 361005, China |
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| Abstract: | A new promising method for determining activity of catalase and its application are presented. The measurement of catalase activity is based on the inhibition of catalase on Fenton reaction. Catalase catalyzes the dissociation of H2O2, one of the main reactants of Fenton reaction which yields ·OH stoichiometrically, and restrains the increment of the fluorescence intensity, produced as a result of quinoline hydroxylation. The decrease of fluorescence intensity was linearly related to the activity of catalase and the linear range covered from 1.7×10-3 U/mL to 1.7×10-2 U/mL. A detection limit of 8.5×10-4 U/mL catalase was obtained. This method has been applied to the detection of catalase in marine biosamples and satisfactory results were obtained. |
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| Keywords: | Catalase determination Quinoline Fluorometry Fenton reaction Biosamples |
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