| SERS标记纳米粒子用于免疫识别 |
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| 引用本文： | 徐抒平,王连英,徐蔚青,赵冰,袁航,马岚,白玉白,樊玉国.SERS标记纳米粒子用于免疫识别[J].高等学校化学学报,2003,24(5):900-902. |
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| 作者姓名： | 徐抒平 王连英 徐蔚青 赵冰 袁航 马岚 白玉白 樊玉国 |
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| 作者单位： | 1. 吉林大学超分子结构与材料教育部重点实验室;
2. 吉林大学化学学院, 长春 130023;
3. 云南大学单克隆抗体工程技术研究中心, 昆明 650091 |
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| 基金项目： | 国家自然科学基金(批准号:29975011,20273022) |
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| 摘 要： | 激光拉曼光谱技术近年来已成为研究生物分子结构常用的光谱手段.尤其在研究水溶液中蛋白质的结构和构象方面发挥了重要作用.然而,常规拉曼光谱的信号强度很低,限制了其在各个领域中的应用.表面增强拉曼光谱(SERS)和表面增强共振拉曼光谱(SERRS)技术可使信号增强6～10个数量级,尤其是SERS技术已发展到检测单分子的水平,更为其在生物方面的应用开拓了新的局.
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| 关 键 词： | SERS 纳米粒子 免疫 标记 |
| 文章编号： | 0251-0790(2003)05-0900-03 |
| 收稿时间： | 2003-01-13 |
Immunological Identification with SERS-labeled Immunogold Nanoparticles by Silver Staining |
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| XU Shu-Ping,WANG Lian-Ying,XU Wei-Qing,ZHAO Bing YUAN Hang,MA Lan,BAI Yu-Bai,FAN Yu-Guo.Immunological Identification with SERS-labeled Immunogold Nanoparticles by Silver Staining[J].Chemical Research In Chinese Universities,2003,24(5):900-902. |
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| Authors: | XU Shu-Ping WANG Lian-Ying XU Wei-Qing ZHAO Bing YUAN Hang MA Lan BAI Yu-Bai FAN Yu-Guo |
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| Institution: | 1. Key laboratory for Supramolecular Structure and Material of Minisry of Education of China ;
2. College of Chemistry, Jilin University, Changchun 130023, China;
3. Monoclonal Antibody Technology Center, Yunnan University, Kunming 650091, China |
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| Abstract: | A spectral measurement combined with nanoparticle labeling and SERS technique is described. The gold nanoparticles not only modified by the mouse monoclonal antibody agaist Hepatitis B surface antigen IgG(H)] but also labeled by the p-mercaptobenzoic acid (MBA) were used as a probe. The immunogold nanoparticles specially react with the antibody against IgG(H)anti IgG(H)] which was immobilzed on the quartz substrates. The identification between the immunogold colloids and the anti IgG(H) was examined by surface enhanced Raman specta(SERS) of MBA after and before silver staining and confirmed by UV-Vis absorption spectra. |
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| Keywords: | Surface enhanced Raman spectroscopy(SERS) Nanoparticle Immunization Labeling |
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