| 纳米ZnO对白僵菌孢子的紫外保护及与孢子的生物相容性 |
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| 引用本文： | 陈培荣,王滨,姚洪章,林华峰,黄勃,李增智.纳米ZnO对白僵菌孢子的紫外保护及与孢子的生物相容性[J].高等学校化学学报,2010,31(12):2322-2328. |
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| 作者姓名： | 陈培荣 王滨 姚洪章 林华峰 黄勃 李增智 |
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| 作者单位： | 1. 安徽农业大学微生物防治安徽省重点实验室, 合肥 230036
2. 安徽农业大学微理学院应用化学系, 合肥 230036
3. 安徽农业大学植物保护学院, 合肥 230036 |
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| 基金项目： | 国家“八六三”计划项目(批准号:2006AA10A212); 国家自然科学基金(批准号:30972368); 安徽省教育厅科研项目(批准号:KJ2007B232)资助 |
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| 摘 要： | 用沉淀法制备经油酸表面修饰的纳米ZnO，测定其紫外吸收性能及对油悬浮剂型白僵菌孢子的抗紫外保护效率；用扫描电子显微镜观察纳米ZnO与孢子的混合状态，通过对含纳米ZnO的白僵菌制剂在贮存期间孢子萌发率差异显著性的分析，确定其与白僵菌孢子的生物相容性.结果表明：制备的纳米ZnO的平均粒径为27nm，其对波长200nm～370nm范围的紫外光具有良好的吸收性能.含纳米ZnO质量分数为1.0%、2.0%和5.0%的油悬浮剂型白僵菌，紫外灯照射8h，对孢子保护效率均大于60%，不含纳米ZnO为9.7％. 纳米ZnO被吸附包覆于孢子表面，屏蔽紫外线，保护孢子免受紫外线的杀伤.含0.5%、1.0%和2.0%纳米ZnO的油悬浮剂型白僵菌制剂室温贮存10个月，其孢子萌发率与不含纳米ZnO的差异不显著；纳米ZnO与白僵菌孢子生物相容性好.油悬浮剂型白僵菌制剂中纳米ZnO的适宜含量为1.0%～2.0%.
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| 关 键 词： | 纳米ZnO 白僵菌 抗紫外剂 生物相容性 |
| 收稿时间： | 2010-06-30 |
Protection of ZnO Nanoparticles to Beauveria bassiana Conidia from Ultraviolet Radiation and Their Biocompatibility |
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| CHEN Pei-Rong,WANG Bin,YAO Hong-Zhang,LIN Hua-Feng,HUANG Bo,LI Zeng-Zhi.Protection of ZnO Nanoparticles to Beauveria bassiana Conidia from Ultraviolet Radiation and Their Biocompatibility[J].Chemical Research In Chinese Universities,2010,31(12):2322-2328. |
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| Authors: | CHEN Pei-Rong WANG Bin YAO Hong-Zhang LIN Hua-Feng HUANG Bo LI Zeng-Zhi |
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| Institution: | 1. Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China;
2. Department of Applied Chemistry, School of Science, Anhui Agricultural University, Hefei 230036, China;
3. College of Plant Protection, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | ZnO nanoparticles modified with oleic acid were prepared by chemical precipitation method,and their ultraviolet absorption properties and protection efficiency to Beauveria bassiana conidia in oil formulation were investigated.The mixture of ZnO nanoparticles and conidia were observed with SEM,and their biocompatibility was evaluated,based on significant difference levels which were analyzed by measuring germination percentage of conidia in formulation after storage.The results show that the obtained ZnO na... |
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| Keywords: | ZnO nanoparticle Beauveria bassiana UV protection agent Biocompatibility |
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