人体乳腺癌组织红外光谱特征的研究

利用红外光谱法对正常乳腺组织、良性肿瘤和乳腺癌组织进行了对比研究.与正常组织的光谱相比,癌组织中蛋白质的某些氨基酸残基的ν_C-O(H)谱带位置明显向高波数位移,表明组织中该基团中的氢键大部分被破坏.蛋白质分子的氢键化的ν_NH谱带位置向低波数位移,且谱带形状也有所改变,说明NH…O=C氢键化程度增强.核酸的磷酸二酯基团的ν_s,PO₂^-谱带吸收强度明显增强,反映癌细胞内DNA相对含量增加;位于970cm^-1附近的ν_s,PO₃^{-谱带强度增加,提示细胞中磷酸化蛋白含量增加.而胶原蛋白的特征谱带强度减弱,说明其相对含量减少.研究证明,红外光谱可在分子水平上揭示乳腺肿瘤的特征.}

Studies on Human Breast Cancer Tissue With Infrared Spectrometry

The normal, benign and malignant human breast tissues have been studied by means of infrared spectroscopy. Some remarkable spectral differences (shape, peak positions and relative intensities) are observed among these three types of the samples. In the malignant breast tissues, these mainly characteristic changes involve: (1) In comparison with the normal breast tissues, the band near 1162 cm^-1 from the stretching modes of C-O(H) group in proteins evidently shifts to a higher wavenumber, which suggests that the intermolecular hydrogen bonds are almost broken; The band near 3310 cm^-1 from the stretching modes of the hydrogen bonded N-Hin protein molecules shifts to a lower wavenumber, which indicates that the intensity of the hydrogen bonds on the NH…O=Cis stronger in protein molecules of malignant breast tissues. (2) The intensity of the symmetric stretching modes of νPO₂^- at 1080 cm^-1 from the phosphodiester groups in cellular nucleic acids is remarkably stronger, which suggests that the relative contents of DNAin cancer cellular increase. The symmetric stretching modes of PO₃^- at 970 cm^-1 from the phosphorylated proteins become stronger, which suggests that the relative contents of phosphorylated protein increase. (3) The change of the intensity of collagen bands indicates the increase of its relative contents. This investigation shows that Infrared spectrometry can represent the characteristic of the breast tumor at the molecular level.