含氮杂冠醚和核酸碱基双亲聚合物的合成及性能

设计合成了含氮杂冠醚和胸腺嘧啶的双亲聚合物聚N,N-二乙氧基-1,10-二氮杂-18冠-6-5-甲基-胸腺嘧啶-异酞酸酯](PCTSE). 用SEM观测到其在水溶液中自发聚集成直径为150~220 nm的纳米球; 用动态光散射测得PCTSE纳米球水溶液的粒径分布主要集中在130~240 nm, 用FTIR研究了PCTSE/腺嘌呤中胸腺嘧啶与底物腺嘌呤的分子识别作用, 结果表明, 聚合物中胸腺嘧啶环上C₄=O伸缩振动峰从1670 cm^-1位移至1664 cm^-1, 表明胸腺嘧啶与腺嘌呤间形成了氢键. 变温红外光谱表明, 该峰又随温度的升高逐渐向高波数位移, 最后位移到识别前的1670 cm^-1处, 表明所形成的氢键断裂.

Synthesis and Property of an Amphiphilic Polymer Containing Nucleobases and Aza-crown Ethers

An amphiphilic polymer containing thymine and aza-crown ethers, poly-{N,N-bis(2-ethoxy)-1,10-diaza-18-crown-6]5-methylene-(1-thymine)] sophthaloyl ester}(PCTSE), was synthesized. The SEM morphology observed via indicates that the amphiphilic PCTSE polymer self-organized into diameters of about 150—220 nm nanospheres in dilute aqueous solution spontaneously. The sizes of PCTSE nanospheres in dilute aqueous solution determined by DLS are mainly dispersed at 130—240 nm. The molecule recognition based on hydrogen bond between thymine of polymer and adenine substrate in PCTSE/adenine nanospheres was stu-died. After recognition the C4=O stretching vibration at 1670 cm-1 of thymine of polymer shifted to 1664 cm-1, which implied the formation of hydrogen bonds between thymine and adenine. The study on variable temperature FTIR show that the peak gradually shifted back to the position at 1670 cm-1, which indicates that the H-bond was broken.