表面接枝季铵盐型高分子材料抗菌过程的特性研究

考察了一种表面接枝季铵盐型高分子材料对水溶液中大肠杆菌的抗菌过程特性,发现此材料可以在短时间内迅速降低溶液中的大肠杆菌的活菌量.用菌体耗氧测定法与扫描电镜观察法研究了其抗菌过程.结果表明,其抗菌过程由吸附和杀灭两个步骤构成,其中吸附过程由纤维与大肠杆菌表面静电作用所控制,是一个快速过程,而杀灭过程则伴随着细胞壁在纤维表面的溶解,是一个慢过程.菌体耗氧测定法与扫描电镜观察法所获得的结果具有一致性,并可广泛应用于研究抗菌纤维的抗菌作用机理及过程特性.

Characterization of the Disinfection Process Using Quaternary Ammonium Salts Grafted Cellulose Fiber

Characterization of disinfection process using quaternary ammonium salts grafted cellulose fiber was carried out with E.coli as a model bacteria. A rapid adsorption of E.coli. on the grafted fiber was identified, as indicated by the dramatic reduction of the concentration of viable cells in the suspensions. The adsorption as function of the fiber input indicates the dominating role of the electrostatic interaction between the fiber and E.coli. cell surface. The determination of oxygen uptake rate (OUR) was introduced to characterize the growth of E.coli. on the fiber surface. The adsorption of E.coli. on the fiber surface was observed with scanning electron microscope(SEM), which showed the lysis of cell envelope along with the processing. The results obtained in the present study show that the disinfection process is composed by two steps, in which the first step, i.e., the adsorption of E.coli. on the fiber surface driven by electrostatic interaction, is a fast reaction. While cell lysis, the second step, is a relatively slow reaction. The present study also shows the potential of OUR and SEM as fundamental tools in the development of antibacterial materials.