壳聚糖微图形的制备及表征

通过微转移模塑法,在硅片表面制备了圆形点阵、方形点阵、沟槽和波浪等4种形貌的壳聚糖(CS)微图形.CS微图形清晰规整,成型效果良好.成骨细胞在圆形点阵和方形点阵上借助伪足多攀附于点阵凸起处.点阵图形对细胞增殖和分化无影响.在沟槽和波浪微图形中,成骨细胞大多被限制在沟区,且沿着沟槽方向伸展.沟槽和波浪微图形不利于细胞分化,但能促进细胞增殖.

Preparation and Characterization of the Chitosan Micropatterns

Micro-transfer molding was used to prepare four different chitosan(CS) micropatterns on silicon substrates: cylinder, square, groove and sine wave. The as-prepared CS micropatterns were well-formed. Osteoblasts were cultured on various micropatterns to evaluate the effects of micro-geometry on cell behavior. On micro-cylinders and micro-squares, osteoblasts attached on CS micro-dot arrays and displayed irregular morphologies with numerous filopodia-like extensions. These micro-dot arrays had no impact on cell proliferation and differentiation. On micro-grooves and sine wave micropatterns, cells located within the channels and aligned to the direction of channels. Micro-grooves and sine wave micropatterns were unfavorable to cell differentiation, whereas promoted cell proliferation.