磷酸钙陶瓷颗粒微纳米结构对蛋白吸附的影响

用微波烧结和常规烧结方法分别制备了4种具有纳米和微米结构的磷酸钙陶瓷, 对陶瓷的相组成、 微观结构、 粒度分布、 比表面积、 孔径分布和表面Zeta电位进行了对比分析, 并进一步采用凝胶电泳法考察了陶瓷对牛血清白蛋白/溶菌酶双蛋白的吸附行为. 结果显示, 纳米陶瓷和常规陶瓷具有相似的相组成、 颗粒分布和表面Zeta电位, 但微孔结构、 比表面积和蛋白吸附差异明显. 纳米陶瓷具有较小的晶粒尺寸和更丰富的介孔结构, 使其能吸附更多的牛血清白蛋白和溶菌酶, 表明其具有更强的生物活性.

Effects of Microstructure of Calcium Phosphate Ceramics Particles on Protein Adsorption

To investigate the effects of physical and chemical properties of nano calcium phosphate ceramics on protein adsorption,two types of nano calcium phosphate particles and two kinds of micron ones were prepared with microwave sintering method and conventional method,respectively.The phase compositions,surface morphologies,particle size distribution,specific surface area,pore size distribution and zeta potentials of four particles were analyzed with X-ray diffraction,scanning electron microscope,dynamic laser system,BET method and zetasizer.Bovine serum album(BSA) and lysozyme(LSZ) adsorbed on ceramics were further investigated by polyacrylamide gel electrophoresis method.The results show that nano and conventional ceramics have similar phase compositions,particle size distribution and zeta potentials.Owing to smaller grain size and more abundant mesoporous structure,nano ceramics adsorb more BSA and LSZ than conventional ones.