| 药物与细胞膜相互作用的ppKCE动力学参数 |
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| 引用本文： | 夏之宁,李丽仙,陈华,熊彩侨,杨丰庆.药物与细胞膜相互作用的ppKCE动力学参数[J].高等学校化学学报,2011,32(4):851-856. |
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| 作者姓名： | 夏之宁 李丽仙 陈华 熊彩侨 杨丰庆 |
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| 作者单位： | 重庆大学生物工程学院;重庆大学化学化工学院;中国科学院化学研究所; |
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| 基金项目： | 国家自然科学基金(批准号:20775096,20805060)资助 |
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| 摘 要： | 在区段-区段动力学毛细管电泳(ppKCE)的理论基础上,以兔红细胞膜和人红细胞膜为受体,研究了药物跨膜转运的动力学过程,同时测得了表征药物与细胞膜间结合快慢的正向结合速率常数kon、反向解离速率常数koff及配体与受体间相互作用的结合常数Kb.考察了迁移时间及峰高的稳定性、pH值和温度对药物与细胞膜相互作用动力学参数的...
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| 关 键 词： | 区段-区段动力学毛细管电泳(ppKCE) 细胞膜 动力学参数 相互作用 |
| 收稿时间： | 2010-07-13 |
Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE |
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| XIA Zhi-Ning,LI Li-Xian,CHEN Hua,XIONG Cai-Qiao,YANG Feng-Qing.Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE[J].Chemical Research In Chinese Universities,2011,32(4):851-856. |
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| Authors: | XIA Zhi-Ning LI Li-Xian CHEN Hua XIONG Cai-Qiao YANG Feng-Qing |
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| Institution: | XIA Zhi-Ning1,2,LI Li-Xian1,CHEN Hua2,XIONG Cai-Qiao3,YANG Feng-Qing2 (1.College of Bioengineering,2.College of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400030,China,3.Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China) |
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| Abstract: | Based on the theory of plug-plug kinetic capillary electrophoresis(ppKCE),the rabbit red blood cell membrane and human red blood cell membrane were used to study the kinetic parameters of the interaction between the cell membrane and drug.The rate constants of complex formation kon,the dissociation koff and the binding constant Kb between cell membrane and drugs were directly determined simultaneously.The stability of peak height and migration time was studied.The influence of pH and temperature on the kine... |
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| Keywords: | Plug-plug kinetic capillary electrophoresis(ppKCE) Cell membrane Kinetic parameter Inte-raction |
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