| 含大环多胺的两亲性小分子的设计合成及与DNA的相互作用 |
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| 引用本文： | 马丽芳,黄清东,张骥,吴江,余孝其.含大环多胺的两亲性小分子的设计合成及与DNA的相互作用[J].高等学校化学学报,2011,32(4):874-878. |
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| 作者姓名： | 马丽芳 黄清东 张骥 吴江 余孝其 |
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| 作者单位： | 四川大学化学学院, 绿色化学与技术教育部重点实验室, 成都 610064 |
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| 基金项目： | 国家自然科学基金(批准号:20972104,20725206和20732004)资助 |
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| 摘 要： | 本文设计合成了两个含大环多胺亲水基团和甾体亲脂结构的两亲性小分子化合物, 化合物的结构经过核磁共振谱, 红外光谱和高分辨质谱确证. 凝胶电泳实验显示这类化合物与Sn-甘油-1,2-二油酰-3-磷酰乙醇胺 (DOPE)形成的阳离子脂质体对DNA有很强包裹能力. 两种脂质体M1和M2分别在N/P比为7和5时即可完全包裹质粒DNA. 另外, 这类脂质体与DNA形成的复合物的粒径经测定在160~220 nm之间, zeta电位为+20~40 mV. 实验结果显示这类脂质体具备了作为非病毒基因载体的潜在性, 可为设计阳离子脂质提供新思路.
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| 关 键 词： | 大环多胺 合成 阳离子脂质 基因载体 |
| 收稿时间： | 2010-06-02 |
Cyclen-based Cationic Lipids:Design,Synthesis and Interactions with DNA |
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| MA Li-Fang,HUANG Qing-Dong,ZHANG Ji,WU Jiang,YU Xiao-Qi.Cyclen-based Cationic Lipids:Design,Synthesis and Interactions with DNA[J].Chemical Research In Chinese Universities,2011,32(4):874-878. |
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| Authors: | MA Li-Fang HUANG Qing-Dong ZHANG Ji WU Jiang YU Xiao-Qi |
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| Institution: | Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China |
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| Abstract: | The clinical success of gene therapy requires a safe and effective gene delivery system.Cationic lipids have particularly excellent potential for gene delivery applications because of their lesser immunogenic nature,ability to deliver large pieces of DNA,and ease of handling and preparation techniques.In this work,two cyclen-based amphiphilic molecules including steroid moieties were designed and synthesized,and their structures were characterized by 1H NMR,IR and HRMS.Cationic liposomes were prepared by mi... |
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| Keywords: | Cyclen Synthesis Cationic lipid Gene vector |
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