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N-羟乙基-1,8-萘二酰亚胺探针与核苷间电子转移的激光闪光光解研究
引用本文:李慧卿,江致勤,王欣,潘洋,王峰,俞书勤.N-羟乙基-1,8-萘二酰亚胺探针与核苷间电子转移的激光闪光光解研究[J].高等学校化学学报,2004,25(11):2134-2136.
作者姓名:李慧卿  江致勤  王欣  潘洋  王峰  俞书勤
作者单位:1. 同济大学化学系,上海,200092
2. 中国科技大学化学物理系,合肥,230026
基金项目:国家自然科学基金 (批准号 :2 0 2 72 0 42 ,2 0 2 73 0 66)资助
摘    要:核酸与核酸前体参与的电子转移(ET)作用能够直接或间接导致核酸主链和碱基侧链的断裂,因此对核酸碱基光动态损害机理的深入研究具有重要的理论和实际意义.其中,核酸荧光探针逐渐成为研究生物分子的主要技术之一,借助于时间分辨的瞬态吸收光谱技术,检测荧光探针激发态物种及其与核酸之间发生电子转移作用而产生的活性中间体,能够深入了解光断裂反应的最初步骤,揭示核酸断裂电子转移反应的微观机理.

关 键 词:萘二酰亚胺  核苷  2-脱氧鸟苷  电子转移  激光闪光光解
文章编号:0251-0790(2004)11-2134-03
收稿时间:2003-08-21

Electron Transfer Laser Flash Photolysis Between Nucleosides and Probe Triplet N-(2'-Hydroxyethyl)-1,8-naphthalimide
LI Hui-Qing,JIANG Zhi-Qin ,Wang Xin,PAN Yang,WANG Feng,YU Shu-Qin.Electron Transfer Laser Flash Photolysis Between Nucleosides and Probe Triplet N-(2''''-Hydroxyethyl)-1,8-naphthalimide[J].Chemical Research In Chinese Universities,2004,25(11):2134-2136.
Authors:LI Hui-Qing  JIANG Zhi-Qin  Wang Xin  PAN Yang  WANG Feng  YU Shu-Qin
Institution:LI Hui-Qing1,JIANG Zhi-Qin 1*,Wang Xin1,PAN Yang2,WANG Feng2,YU Shu-Qin2
Abstract:The time resolved transient absorption property, kinetics and mechanism of electron transfer(ET) process of the fluorescence probe, N-(2-hydroxyethyl)-1,8-naphthalimide(NPI) were studied. The transient absorption spectra of NPI in H2O-CH3CN solution were successfully detected upon pulsed excitation at 355 nm. 3NPI was proved as the primary excited state with lifetime of 147 μs. 3NPI underwent the T-T energy transfer with β-carotene and ET with triphenyl amine to produce NPI-·. In the absence of quenchers, the self-quenching of 3NPI by the ground states of NPI took place via ET. The ET from nucleosides to 3NPI was also explored. NPI showed a strong electron-accepting capability and the absorbance of 3NPI was strongly quenched by the nucleosides. The formation of NPI-· was facilitated to a great extent simultaneously. The bimolecular quenching rate constants of NPI by GUA and DGUA were determined as k3=8.8×106mol-1·L·s-1, k4=2.1×107 mol·L·s-1. The ET between NPI and nucleosides was verified and the mechanism was proposed.
Keywords:Naphthalimide  Nucleoside  2'-Dioxyguanosine  Electron transfer  Laser flash photolysis
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