染料壳聚糖微球的制备及其对牛血清白蛋白(BSA)吸附性能的研究

采用反相悬浮交联法制备壳聚糖微球,对微球进行羟丙基氯化及氨基化,并偶联色素配体Cibacron Blue F3GA,得到一种新型染料亲和吸附剂.以牛血清白蛋白(BSA)为目标蛋白,考察了该染料亲和吸附剂的吸附性能,发现其对BSA有较高的吸附量(95.2mg/g),吸附行为满足Langmuir吸附等温式.负载牛血清白蛋白的微球容易洗脱,洗脱率高达99％.

Preparation of Cibacron Blue F3GA Attached Chitosan Microspheres and Their Adsorption Properties for Bovine Serum Albumin(BSA)

A series of novel aminated chitosan microbeads were prepared from chitosan through inverse suspension crosslinking,hydroxylpropyl-chlorination and amination.Affinity dye-ligand,Cibacron Blue F3GA,was covalently coupled with the aminated chitosan microspheres via space arm between the triazine ring of Cibacron Blue F3GA and the amino groups of the microspheres.The maximum attachment of Cibacron Blue F3GA was 494 μmol/g by changing the crosslinking degree of the microspheres.Albumin adsorption onto Cibacron Blue F3GA-attached chitosan microspheres was investigated.The BSA equilibrium adsorption capacity was 95.2 mg/g dry beads.The adsorption phenomena appeared to follow a typical Langmuir isotherm.The highest desorption ratio(over 99%) was achieved by using 1.0 mol/L NaSCN(pH=8.0).The Cibacron Blue F3GA-attached chitosan microspheres could be reused without significant decreases in the adsorption capacities.