| 光谱法研究硫鸟嘌呤与七元瓜环及牛血清白蛋白的超分子相互作用 |
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| 引用本文： | 黄英,王娟,郭改英,陶朱,薛赛凤,祝黔江,周清娣.光谱法研究硫鸟嘌呤与七元瓜环及牛血清白蛋白的超分子相互作用[J].高等学校化学学报,2013,34(2):375-380. |
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| 作者姓名： | 黄英 王娟 郭改英 陶朱 薛赛凤 祝黔江 周清娣 |
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| 作者单位： | 1. 贵州大学西南药用生物资源教育部工程研究中心;
2. 贵州省大环化学及超分子化学重点实验室, 贵阳 550025;
3. 悉尼大学化学院, 澳大利亚新南威尔士州 2006 |
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| 基金项目： | 国家自然科学基金(批准号:20972034);贵州省教育厅自然科学基金(批准号:黔教科2010013号);贵州省科技厅国际合作项目(批准号:黔科合外G字[2012]7003号);教育部春晖计划项目(批准号:Z2011034)资助 |
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| 摘 要： | 利用紫外-可见吸收光谱法和荧光光谱法研究了抗癌药物硫鸟嘌呤(6-TG)与七元瓜环(Q7])及牛血清白蛋白(BSA)的相互作用. 结果表明, 6-TG与Q7]及BSA可形成三元复合物, 且6-TG与Q7]及BSA均可形成1:1的超分子配合物, 6-TG能引起BSA的荧光猝灭, 猝灭机制为静态猝灭. 此外, 还用同步荧光法和三维荧光法考察了6-TG对BSA构象的影响, 结果表明6-TG的加入使BSA的构象发生了变化, 而同步荧光光谱结果表明结合位点更接近于色氨酸.
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| 关 键 词： | 硫鸟嘌呤 七元瓜环 牛血清白蛋白 紫外-可见吸收光谱 荧光光谱 |
| 收稿时间： | 2012-07-06 |
Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method |
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| HUANG Ying,WANG Juan,GUO Gai-Ying,TAO Zhu,XUE Sai-Feng,ZHU Qian-Jiang,ZHOU Qing-Di.Interaction of 6-Thioguanine with Cucurbit[7]uril and Bovine Serum Albumin by Spectroscopic Method[J].Chemical Research In Chinese Universities,2013,34(2):375-380. |
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| Authors: | HUANG Ying WANG Juan GUO Gai-Ying TAO Zhu XUE Sai-Feng ZHU Qian-Jiang ZHOU Qing-Di |
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| Institution: | 1. The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources, Ministry of Education;
2. Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025, China;
3. School of Chemistry, the University of Sydney, NSW 2006, Australia |
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| Abstract: | The aim of the present study was to investigate the interaction of 6-thioguanine(6-TG) with cucurbit7]uril(Q7]) and bovine serum albumin(BSA) in an acetate buffer solution by ultraviolet absorption spectroscopy, fluorescence emission spectroscopy and synchronous fluorescence spectra, as well as and three-dimensional fluorescence spectra. It was indicated from UV-Vis spectra that 6-TG-Q7] binary complex formed. The fluorescence quenching of BSA by 6-TG was a result of the formation of 6-TG-BSA complex, and the quenching mechanism of BSA by 6-TG was static quenching mechanism. Moreover, effect of 6-TG on BSA conformation was discussed by the studies of synchronous and three-dimensional fluorescence spectra. In addition, a specific binding interaction took place between the 6-TG and Q7] in the presence of BSA. |
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| Keywords: | 6-Thioguanine Cucurbit[7]uril Bovine serum albumin Ultraviolet absorption spectroscopy Fluorescence spectroscopy |
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