正负离子表面活性剂与高聚物混合双水相体系的相行为及蛋白质的分配

报道了由正负离子表面活性剂与高聚物混合溶液形成的一种可用于蛋白质的分离及分析的新型双水相萃取体系.研究了正负离子表面活性剂(溴化十二烷基三乙铵/十二烷基硫酸钠)分别与葡聚糖和聚乙二醇混合双水相体系的形成规律、相行为及牛血清蛋白和溶菌酶在双水相体系中的分配.通过在高聚物分子中接上亲和配基,研究蛋白质在双水相体系中的亲和分配.结果表明,在该体系中,表面活性剂与高聚物分别富集于不同相中.升高温度及加入无机盐均可促进双水相体系的形成,不同蛋白质可分配于不同的相中.亲和配基的引入极大地增强了蛋白质分配的选择性.

Phase Behavior and Protein Partitioning in Aqueous Two-phase Systems Formed by Mixtures of Polymer and Cationic-anionic Surfactants

A novel aqueous two-phase system can be formed by the mixtures of a polymer and cationic-anionic surfactants. Such a system can be used as a partitioning system of proteins. In this work, we investigated the formation, phase behavior and protein partitioning in aqueous two-phase systems formed by dodecyltriethylammonium bromide/sodium dodecylsulfate/PEG and dodecyltriethylammonium bromide/sodium dodecylsulfate/dextran. The ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. It was shown that the surfactants and polymers are enriched in different phases of aqueous two-phase systems. Phase separation are promoted by increasing temperature and adding inorganic salts. Different proteins are partitioned in different phases. The selectivity of protein partitioning is increased by adding ligands with affinity.