| 纳米电极-膜片钳监测单个活囊泡连续释放 |
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| 引用本文： | 吴文展,黄卫华,王玮,王宗礼,程介克,张蓉颖,陈宇,刘杰,郑从义,沈超.纳米电极-膜片钳监测单个活囊泡连续释放[J].高等学校化学学报,2005,26(5):829-831. |
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| 作者姓名： | 吴文展 黄卫华 王玮 王宗礼 程介克 张蓉颖 陈宇 刘杰 郑从义 沈超 |
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| 作者单位： | 1. 武汉大学化学与分子科学学院, 武汉430072;
2. 华中科技大学生命科学与技术学院, 武汉430074;
3. 武汉大学生命科学院, 武汉430072 |
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| 摘 要： | Carbon fiber nanoelectrode(tip diameter ca. 100nm)-patch clamp was firstly applied to realtime monitoring dopamine release from single living vesicles of single Rat pheochromocytoma (PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis.
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| 关 键 词： | 纳米电极 膜片钳 单囊泡空间分辨 连续囊泡释放 |
| 文章编号： | 0251-0790(2005)05-0829-03 |
| 收稿时间： | 2005-02-03 |
Nanoelectrode-patch Clamp Monitoring of Continual Single Living Vesicle Release |
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| WU Wen-Zhan,Huang Wei-hua,WANG Wei,WANG Zong-li,Cheng Jie-ke,ZHANG Rong-Ying,CHEN Yu,LIU Jie,ZHENG Cong-yi,SHEN Chao.Nanoelectrode-patch Clamp Monitoring of Continual Single Living Vesicle Release[J].Chemical Research In Chinese Universities,2005,26(5):829-831. |
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| Authors: | WU Wen-Zhan Huang Wei-hua WANG Wei WANG Zong-li Cheng Jie-ke ZHANG Rong-Ying CHEN Yu LIU Jie ZHENG Cong-yi SHEN Chao |
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| Institution: | 1. College of Chemistry & Molecular Science, Wuhan University, Wuhan 430072, China;
2. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China;
3. College of Life Science, Wuhan University, Wuhan 430072, China |
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| Abstract: | Carbon fiber nanoelectrode(tip diameter ca. 100 nm)-patch clamp was firstly applied to real-time monitoring dopamine release from single living vesicles of single Rat pheochromocytoma(PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis. |
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| Keywords: | Nanoelectrode Patch clamp Single vesicle spatial resolution Continual vesicle release |
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