| 电离辐射诱导T淋巴细胞的差异蛋白质组学研究 |
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| 引用本文： | 梁峰,石莹,张萱,刘忠英,刘志强.电离辐射诱导T淋巴细胞的差异蛋白质组学研究[J].高等学校化学学报,2010,31(6):1143-1147. |
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| 作者姓名： | 梁峰 石莹 张萱 刘忠英 刘志强 |
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| 作者单位： | 1. 吉林大学药学院, 长春 130021;
2. 中国科学院长春应用化学研究所, 长春质谱中心, 长春 130022;
3. 吉林大学公共卫生学院, 长春 130021 |
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| 摘 要： | 应用电离辐射诱导人类T淋巴细胞白血病Jurkat细胞株, 再经二维凝胶电泳分离不同照射剂量的细胞总蛋白, 研究电离辐射诱导的人类T淋巴细胞白血病细胞蛋白的差异表达. 应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)进行处理, 得到肽质量指纹(PMF)图谱, 确认6个差异表达蛋白点.
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| 关 键 词： | 蛋白质组 二维凝胶电泳 基质辅助激光解吸电离飞行时间质谱 Jurkat细胞 |
| 收稿时间： | 2009-11-02 |
Differential Proteomic Study of Human T Lymphocytic Leukemia Protein Induced by Ionizing Radiation |
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| LIANG Feng,SHI Ying,ZHANG Xuan,LIU Zhong-Ying,LIU Zhi-Qiang.Differential Proteomic Study of Human T Lymphocytic Leukemia Protein Induced by Ionizing Radiation[J].Chemical Research In Chinese Universities,2010,31(6):1143-1147. |
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| Authors: | LIANG Feng SHI Ying ZHANG Xuan LIU Zhong-Ying LIU Zhi-Qiang |
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| Institution: | 1. School of PharmaceuticalSciences, Jilin University, Changchun 130021, China;
2. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
3. School of Public Health, Jilin University, Changchun 130021, China |
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| Abstract: | Two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrorometry(MALDI-TOF-MS) have been acknowledged as the efficient methods in proteomics studies.In this report,the total proteins of human T lymphocytic leukemia(Jurkat cells) treated by ionizing radiation were separated with two-dimensional electrophoresis.The differential expression of cellular proteins induced by ionizing radiation was analyzed by PDQUEST software.Six differential protein spots were ide... |
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| Keywords: | Proteome Two-dimensional electrophoresis Matrix-assisted laser desorption/ionization time-of-flight mass spectrorometry(MALDI-TOF-MS) Jurkat cell |
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