柔红霉素与DNA作用的序列特异性研究

采用紫外-可见光谱法和紫外-可见光谱电化学法研究了柔红霉素(DNR)与不同寡聚核苷酸之间的相互作用.结果表明,DNR优先作用于寡聚核苷酸的CpG位点,然后是ApG和ApC.因为DNR可与鸟嘌呤之间形成3个氢键.与双链寡聚核苷酸作用时,DNR最先插入的位点是(CpG)2碱基对之间,其次是(TpG)(CpA)和(GpC)(ApC)碱基对之间.当DNR与存在未配对G碱基的DNA链作用时,因游离的DNR量增加使其电化学活性增加,导致DNA构象和构型的变化,使DNA生理功能受到损伤,DNA碱基增色效应显著上升.此法可用于G碱基未配对DNA链的检测.

Studies on the Interaction of Daunomycin and the Specific Sequence of DNA

On the ground of UV-spectrophotometry and UV-spectroelectrochemistry, the interaction of daunomycin(DNR) and specific sequence of DNA has been investigated. As the results obtained in this paper, DNR can react with the sequence of oligonucleotide which is CpG, ApG and ApC successively; the sequence of base pairs of double helix of DNA which are intercalated by DNR are (CpG) 2, (TpG)(CpA) and (CpT)(ApG). An important conclusion has been gained in this paper that if G bases were not pairing in the helix of DNA, the spectroelectrochemical characteristic of the adducts containing that kind of DNA and DNR would exhibit a remarkable difference with those adducts consisting of DNR and normal DNA. It indicates that this characteristic can be used to detect and determine the DNA helix which has one base unpaired or mispairing. The results obtained in this paper are of clinical and pharmacal importance.