| 金电极表面聚赖氨酸固定微过氧化物酶-11的电化学研究 |
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| 引用本文： | 王凤彬,郑军伟,李晓伟,季媛,高颖,邢巍,陆天虹.金电极表面聚赖氨酸固定微过氧化物酶-11的电化学研究[J].高等学校化学学报,2003,24(12):2268-2270. |
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| 作者姓名： | 王凤彬 郑军伟 李晓伟 季媛 高颖 邢巍 陆天虹 |
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| 作者单位： | 1. 中国科学院长春应用化学研究所电分析化学国家重点实验室, 长春 130022;
2. 苏州大学化学系, 苏州 215006;
3. 哈尔滨师范大学化学系, 哈尔滨 150080 |
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| 基金项目： | 国家自然科学基金 (批准号 :2 0 0 73 0 2 8,2 0 2 43 0 2 2 )，黑龙江省自然科学基金 (批准号 :B2 0 0 0 -0 6)资助 |
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| 摘 要： | 通过聚赖氨酸修饰将微过氧化物酶-11(MP-11)固定在金电极表面,制备成MP-11修饰电极.修饰在电极表面上的MP-11的血红素活性中心与电极之间可进行直接的电子传递反应,其氧化还原式电位为-0.39V.该修饰电极对氧的还原具有电催化活性.当MP-11与咪唑发生轴向配位反应时,其氧化还原式电位发生负移,此时对氧的还原不再具有电催化活性.
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| 关 键 词： | 微过氧化物酶-11 聚赖氨酸 咪唑 电催化 紫外-可见吸收光谱 |
| 文章编号： | 0251-0790(2003)12-2268-03 |
| 收稿时间： | 2002-08-08 |
Electrochemical Study of Microperoxidase-11 Immobilized on the Surface of a Poly-L-lysine Modified Gold Electrode |
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| WAGN Feng-Bin ,ZHENG Jun-Wei ,LI Xiao-Wei ,JI Yuan ,GAO Ying ,XING Wei ,LU Tian-Hong.Electrochemical Study of Microperoxidase-11 Immobilized on the Surface of a Poly-L-lysine Modified Gold Electrode[J].Chemical Research In Chinese Universities,2003,24(12):2268-2270. |
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| Authors: | WAGN Feng-Bin ZHENG Jun-Wei LI Xiao-Wei JI Yuan GAO Ying XING Wei LU Tian-Hong |
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| Institution: | 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Department of Chemistry, Suzhou University, Suzhou 215006, China;
3. Department of Chemistry, Haerbin Normal University, Haerbin 150080, China |
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| Abstract: | MP-11 was immobilized on the surface of the poly-L-lysine modified gold electrode. Direct electron transfer between the heme and the electrode was observed. The formal redox potential for MP-11 immobilized at the gold electrode is -0.39 V in the phosphate buffer solution(pH=7.0). The MP-11 modified electrode possesses the good electrocatalytic activity for the reduction of O 2. When imidazole becomes the sixth ligand, the formal redox potential of MP-11 would shift to the negative direction and the electrocatalytic activity of MP-11 for the reduction of O 2 is significantly inhibited. |
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| Keywords: | Microperoxidase-11 Poly-L-lysine Imidazole Electrocatalysis UV-Vis absorption spectrum |
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